Weak power frequency magnetic fields induce microtubule cytoskeleton reorganization depending on epidermal growth factor receptor & calcium signaling

Abstract

Overview

This study explores the impact of a 50 Hz magnetic field (MF) on the microtubule (MT)-cytoskeleton within various cell types, extending previous research which highlighted potential activations related to the epidermal growth factor receptor (EGFR) pathways enhancing cell motility.

Findings

• The 0.4 mT MF was shown to suppress MT organization across different cell types including PC12 and FL cells.
• MF exposure resulted in similar effects to EGF stimulation, such as promoting clustering of the EGFR and triggering phosphorylation at specific sites.
• The presence of MF effects was also observed to be dependent on calcium signaling facilitated by L-type calcium channel (LTCC) activation and raised intracellular Ca²⁺ levels.
• Notable connections between EGFR and Ca²⁺ signaling paths were identified, underpinning the MF-induced reorganization of the cytoskeleton network through phosphorylation of relevant signaling proteins, including the MT-associated protein tau.

Conclusion

This investigation underscores that weak magnetic fields can significantly influence cellular architecture via complex signaling relationships involving both EGFR and calcium pathways, suggesting risks to cellular integrity and potential implications for health in environments exposed to electromagnetic fields.