Abstract

Overview

Three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed using transformed Escherichia coli (E. coli) cells. These transformations involved plasmids where the expression of a fluorescent protein is controlled by the promotor of genes matA, safA, or chbB.

Methodology

The biosensors were subjected to THz radiation from two sources: a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device.

Findings

• The threshold and dynamics of fluorescence depended on the radiation parameters and exposure duration.
• Under heat shock or chemical stress, there was no induction of fluorescence, indicating specific responses to THz radiation.

Conclusion

The developed biosensors are effective for studying the influence of THz radiation on the activity of gene networks associated with the targeted gene promoters, highlighting potential risks and effects of THz exposure.