Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling

Abstract

Overview

The exploration of synthetic biology continues to reveal groundbreaking methods of interfacing biological systems with electronic devices. This study specifically delves into manipulating cellular behavior and gene expression in Escherichia coli through integrative redox signalling mechanisms.

Findings

Utilizing a novel electrogenetic device, the research demonstrates the ability to convert electronic information into biochemically relevant signals that can actively control gene expression. This is achieved through electronic activation of the native transcriptional regulator, SoxR, and its subsequent control over transcription from the PsoxS promoter.

• Quick and reversible cellular responses were observed.
• The response intensity is dependent on the amplitude and frequency of the imposed electronic signals.
• Capability of inducing bacterial motility and enhancing cell-to-cell communication is shown.

Conclusion

This work not only extends the capabilities of synthetic biology but also contributes significantly to the emergent field of bioelectronics by demonstrating a robust approach for bidirectional communication between electronic devices and biological components. The implications for health and safety in the context of electromotive force exposure and microbial handling are profound and warrant careful consideration.