Glioblastoma Cell Migration is Directed by Electrical Signals

Authors: Hannah Clancy, Michal Pruski, Bing Lang, Jared Ching, Colin D McCaig

Year: 2021 Jul 14

Category: Cell Biology, Oncology

Journal: Exp Cell Res

DOI: 10.1016/j.yexcr.2021.112736

URL: https://pubmed.ncbi.nlm.nih.gov/34273404/

Abstract

Overview

Our study focuses on the significant role electric fields (EFs) play in directing cell migration within the context of glioblastoma multiforme (GBM) and neural stem cells, pointing toward novel intervention strategies for tumor management.

Key Findings

  • EF-directed cell migration (electrotaxis) occurs in both GBM and neural stem cells, with important pathways like EGFR/PI3K/Akt frequently dysregulated.
  • We observed that primary differentiated GBM cells and glioma stem cells (GSCs) show different preferences for migration towards anode and cathode, respectively.
  • The application of the PPARγ agonist, pioglitazone, aimed at disrupting the Akt pathway via PTEN upregulation, resulted in significant inhibition of directed cell migration in both differentiated GBM and GSC subtypes.
  • Western blot analysis confirmed that the expression of PPARγ remained unchanged with and without EF exposure.

Conclusion

The ability to chemically inhibit electrotaxis in GBM cells through pioglitazone introduces a compelling approach to potentially thwart tumor recurrence by targeting GBM electric field responses.

glioblastoma electrotaxis cell migration electric fields PPARγ Akt pioglitazone
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