900 MHz Radiofrequency Field Induces Mitochondrial Unfolded Protein Response in Mouse Bone Marrow Stem Cells

Abstract

Overview

The research investigates whether a 900 MHz radiofrequency field, common in mobile communication, can trigger mitochondrial unfolded protein response (UPR^mt) in mouse bone marrow stromal cells (BMSC).

Methods

• BMSCs were exposed to continuous wave 900 MHz radiofrequency fields at 120 μW/cm² for 4 hours per day over 5 consecutive days.
• Control groups included cells both with and without radiofrequency radiation, including a positive control exposed to X-Ray radiation.
• Another group received siRNA pretreatment to study molecular interactions.

Findings

• Significant, though temporary, increases in reactive oxygen species (ROS) and UPR^mt related proteins were observed shortly after exposure to the fields.
• This change was temporary, and mitochondrial protein folding ability was restored 24 hours post-exposure.

Conclusion

While the 900 MHz RF exposure did increase ROS levels and temporarily activated UPR^mt, it did not cause permanent or severe mitochondrial dysfunction. Further research is needed to fully understand the molecular mechanisms behind RF-induced UPR^mt.